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The novelty of the work is the use of two SPE columns online

The polyamines include 1,3 diaminopropane, putrescine, cadaverin, N acetyl putrescine, spermidine, spermine, N1 acetyl spermine, and l ornithine. The novelty of the work is the use of two SPE columns online coupled to LC/MS/MS, which minimizes the sample pretreatment to a single derivatization step. The analysis is complete within 4min, making the method highly suitable for routine clinical analysis and high throughput screenings.

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